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Anti-CD42b (GPlbα)
糖蛋白Ibα（GPIbα）也称为CD42b，是血小板上GPIb-V-IX复合物的成分。 GPIb-V-IX复合物结合von Willebrand因子，使血小板粘附和血小板栓塞在血管损伤部位形成。 这些抗CD42b单克隆抗体是在杂交瘤细胞中产生的，该杂交瘤细胞是从用野生型小鼠血小板免疫的BALB / cGPIbα基因敲除小鼠中分离出来的。
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抗GPIbα单克隆抗体的表征
Monoclonal antibodies were generated in GPIbα-/- mice (immunized with wild-type platelets). Subtype and binding to platelets were analyzed by flow cytometry using washed platelets pre-incubated with 5 μg/mL of monoclonal antibodies and then incubation with FITC-conjugated anti-mouse IgM, IgG, IgG1, IgG2a, IgG2b, and IgG3 secondary antibodies. M: mouse, P: pig
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